QUANTIFICATION OF MITOCHONDRIAL PROTEINS IN CULTURED-CELLS BY IMMUNO-FLOW CYTOMETRY

被引：13

作者：

VANDENBOGERT, C

PENNINGS, A

DEKKER, HL

LUCIAKOVA, K

BOEZEMAN, JBM

SINJORGO, KMC

机构：

[1] UNIV HOSP NIJMEGEN, DIV HEMATOL, NIJMEGEN, NETHERLANDS

[2] SLOVAK ACAD SCI, CANC RES INST, CS-80936 BRATISLAVA, CZECHOSLOVAKIA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1097卷 / 02期

关键词：

IMMUNO-FLOW CYTOMETRY; IMMUNOPRECIPITATION; MITOCHONDRIAL PROTEIN; CYTOCHROME-C OXIDASE; MITOCHONDRIAL MYOPATHY;

D O I：

10.1016/0925-4439(91)90090-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Immuno-flow cytometry was tested as a tool to estimate the cellular concentration of mitochondrial proteins in cultured cells, using cytochrome c oxidase as a model enzyme. Cells labelled with antibodies against cytochrome c oxidase, in which the amount of the enzyme was reduced by various extents, showed a linear relationship between the size of the signal obtained by immuno-flow cytometry and the amount of the enzyme. The determination by immuno-flow cytometry resulted in data comparable to the results obtained by immunoprecipitation and activity measurements. Since immuno-flow cytometry requires only limited numbers of cells, the method could especially be of value for diagnostic purposes. This is illustrated by the results obtained by comparing activity measurements and immuno-flow cytometry in the initial screening of cell lines derived from patients with deficiencies in the activity of cytochrome c oxidase.

引用

页码：87 / 94

页数：8

共 21 条

[1] ANALYSIS OF PCP-DATA TO DETERMINE FRACTION OF CELLS IN VARIOUS PHASES OF CELL-CYCLE [J].