CLONING OF THE RAT AND HUMAN PROSTAGLANDIN-F2-ALPHA RECEPTORS AND THE EXPRESSION OF THE RAT PROSTAGLANDIN-F2-ALPHA RECEPTOR

被引：67

作者：

LAKE, S ^{[1
]}

GULLBERG, H ^{[1
]}

WAHLQVIST, J ^{[1
]}

SJOGREN, AM ^{[1
]}

KINHULT, A ^{[1
]}

LIND, P ^{[1
]}

HELLSTROMLINDAHL, E ^{[1
]}

STJERNSCHANTZ, J ^{[1
]}

机构：

[1] PHARM OPTHALM,GLAUCOMA RES,S-75182 UPPSALA,SWEDEN

来源：

FEBS LETTERS | 1994年 / 355卷 / 03期

关键词：

PROSTANOID RECEPTOR; PGF(2-ALPHA); CLONING; EXPRESSION; LIGAND BINDING; NORTHERN ANALYSIS;

D O I：

10.1016/0014-5793(94)01198-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned the FP receptor from rat corpus luteum and human uterus cDNA libraries, respectively. The coding DNA sequence in the rat cDNA is 1101 bp and is similar to the mouse cDNA coding for a receptor protein of 366 amino acids. The human sequence shows a 5 bp deficiency in the 3' region, truncating the coding sequence to 359 amino acids. Northern blot analysis indicates highest expression in the ovary. Cell lines have been established giving stable expression of the FP receptor. Activation of the cloned FP receptor gave an increase in intracellular calcium, indicating signaling via phospholipase C-mediated phosphoinositide turnover. Using [H-3]PGF(2 alpha), binding of PGs showed the rank order of fluprostenol > PhXA70 > PGF(2 alpha) greater than or equal to PhXA85 > PGD(2) > PGE(2).

引用

页码：317 / 325

页数：9

共 44 条

[1] PROSTAGLANDIN-F2-ALPHA ACTIVATES PROTEIN-KINASE-C IN HUMAN OVARIAN-CELLS [J].