MAPPING EPITOPES ON A PROTEIN ANTIGEN BY THE PROTEOLYSIS OF ANTIGEN-ANTIBODY COMPLEXES

被引：155

作者：

JEMMERSON, R ^{[1
]}

PATERSON, Y ^{[1
]}

机构：

[1] SCRIPPS CLIN & RES FDN, DEPT IMMUNOL, 10666 N TORREY PINES RD, LA JOLLA, CA 92037 USA

来源：

SCIENCE | 1986年 / 232卷 / 4753期

关键词：

D O I：

10.1126/science.2422757

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A monoclonal antibody bound to a protein antigen decreases the rate of proteolytic cleavage of the antigen, having the greatest effect on those regions involved in antibody contact. Thus, an epitope can be identified by the ability of the antibody to protect one region of the antigen more than others from proteolysis. By means of this approach, two distinct epitopes, both conformationally well-ordered, were characterized on horse cytochrome c.

引用

页码：1001 / 1004

页数：4

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