A RADIOIMMUNOHISTOCHEMICAL METHOD FOR AUTORADIOGRAPHIC VISUALIZATION OF CELL ANTIGENS USING I-125 STAPHYLOCOCCAL PROTEIN-A

被引：3

作者：

CORREA, FMA ^{[1
]}

GUILHAUME, SS ^{[1
]}

SAAVEDRA, JM ^{[1
]}

机构：

[1] NIMH, CLIN SCI LAB,PRECLIN NEUROPHARMACOL UNIT, 9000 ROCKVILLE PIKE,BLDG 10,ROOM 2D46, BETHESDA, MD 20892 USA

来源：

NEUROSCIENCE LETTERS | 1988年 / 89卷 / 02期

关键词：

D O I：

10.1016/0304-3940(88)90368-0

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

125I-Staphylococcal protein A was used to visualize immunoreactive cell antigens in rat brain and pituitary and autoradiography. Autoradiograms of rat brain sections generated with 125I-protein A were clear and showed low background signals. We were able to visualize neural structures containing tyrosine hydroxylase or methionine-enkephalin-like immunoreactivities in the brain, and vasopressin-like immunoreactivity in the pituitary gland. Our results suggest that 125I-protein A can be used for the radioimmunohistochemical visualization of cell antigens in tissue sections.

引用

收藏

页码：127 / 132

页数：6

相关论文

共 30 条

[1] COMPARATIVE SENSITIVITY OF I-125 PROTEIN-A AND ENZYME-CONJUGATED ANTIBODIES FOR DETECTION OF IMMUNOBLOTTED PROTEINS [J].

BERNSTEIN, JM ;

STOKES, CE ;

FERNIE, B .

JOURNAL OF CLINICAL MICROBIOLOGY, 1987, 25 (01) :72-75

[2] DEMONSTRATION AND ASSAYING OF IGG ANTIBODIES IN TISSUES AND ON CELLS BY LABELED STAPHYLOCOCCAL PROTEIN A [J].

BIBERFELD, P ;

GHETIE, V ;

SJOQUIST, J .

JOURNAL OF IMMUNOLOGICAL METHODS, 1975, 6 (03) :249-259

[3] PROTEIN-A IN IMMUNOPEROXIDASE TECHNIQUES [J].

CELIO, MR ;

LUTZ, H ;

BINZ, H ;

FEY, H .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1979, 27 (02) :691-693

[4] IMMUNOCYTOCHEMISTRY WITH INTERNALLY LABELED MONOCLONAL-ANTIBODIES [J].

CUELLO, AC ;

PRIESTLEY, JV ;

MILSTEIN, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (02) :665-669

[5] A CRITICAL-REVIEW OF LIGHT AND ELECTRON-MICROSCOPIC IMMUNOCYTOCHEMICAL TECHNIQUES USED IN NEUROBIOLOGY [J].

DEMEY, J .

JOURNAL OF NEUROSCIENCE METHODS, 1983, 7 (01) :1-18

[6] RADIOIMMUNOASSAY OF CELLULAR SURFACE ANTIGENS ON LIVING CELLS USING IODINATED SOLUBLE-PROTEIN A FROM STAPHYLOCOCCUS-AUREUS [J].

DORVAL, G ;

WELSH, KI ;

WIGZELL, H .

JOURNAL OF IMMUNOLOGICAL METHODS, 1975, 7 (2-3) :237-249

[7] PROTEIN-A - PEROXIDASE - VALUABLE TOOL FOR LOCALIZATION OF ANTIGENS [J].

DUBOISDALCQ, M ;

MCFARLAND, H ;

MCFARLIN, D .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1977, 25 (11) :1201-1206

[8]

FORSGREN A, 1966, J IMMUNOL, V97, P822

[9] RADIOIMMUNOCYTOCHEMISTRY USING A TRITIATED GOAT ANTI-RABBIT 2ND ANTIBODY [J].

GLAZER, EJ ;

RAMACHANDRAN, J ;

BASBAUM, AI .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1984, 32 (07) :778-782

[10] USE OF STAPHYLOCOCCAL PROTEIN-A AS AN IMMUNOLOGICAL REAGENT [J].

GODING, JW .

JOURNAL OF IMMUNOLOGICAL METHODS, 1978, 20 (APR) :241-253