PREPARATIVE AND ANALYTICAL SEPARATION OF OLIGOSACCHARIDES FROM KAPPA-CARRAGEENAN

被引：13

作者：

MALFAIT, T

VANCAUWELAERT, F

机构：

[1] Interdisciplinary Research Centre, Katholieke Universiteit Leuven, B-8500 Kortrijk, Campus Kortrijk

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 504卷 / 02期

关键词：

D O I：

10.1016/S0021-9673(01)89540-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The separation of the enzymic degradation products of κ-carrageenan, as studied by size-exclusion chromatography on Bio-Gel P-6 and reversed-phase high-performance liquid chromatography μBondapak C18, resulted in a homologous series of oligomers in addition to non-homologues substances. On the former column steric exclusion competed with ion exclusion originating from solute-solute and solute-gel interactions. Competition was reduced with increasing ionic strength of the eluent. Separation on μBondapak C18, was influenced by the type of salt, indicating that the reversed-phase separation was not solely based on solute-column interactions. © 1990.

引用

页码：369 / 380

页数：12

共 27 条

[1] CARRAGEENANS .7. POLYSACCHARIDES FROM EUCHEUMA-SPINOSUM AND EUCHEUMA-COTTONII - COVALENT STRUCTURE OF IOTA-CARRAGEENAN [J].