EVALUATION OF ETCHED BOROSILICATE GLASS-CAPILLARY COLUMNS IN REVERSED PHASE OPEN TUBULAR LIQUID-CHROMATOGRAPHY

被引：4

作者：

DOHMEIER, DM ^{[1
]}

JORGENSON, JW ^{[1
]}

机构：

[1] UNIV N CAROLINA,DEPT CHEM,CHAPEL HILL,NC 27599

来源：

JOURNAL OF MICROCOLUMN SEPARATIONS | 1991年 / 3卷 / 04期

关键词：

OPEN TUBULAR LIQUID CHROMATOGRAPHY; CAPILLARY LIQUID CHROMATOGRAPHY; COLUMN EFFICIENCY;

D O I：

10.1002/mcs.1220030404

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The performance of etched borosilicate capillary columns with a bonded octadecyl stationary phase has been examined. Plate heights for three test compounds in columns with inner diameters between 6. 5 and 14.6-mu-m were measured as a function of mobile phase velocity. The reduced plate height vs. reduced velocity curves were then fit to the Golay equation using nonlinear regression analysis. It was found that in addition to longitudinal diffusion in the mobile phase, longitudinal diffusion in the stationary phase was a significant contributor to broadening at low mobile phase flow rates. At higher flow rates, resistance to mass transfer in the stagnant mobile phase in the porous glass produced by the etching procedure became measurable.

引用

页码：311 / 317

页数：7

共 28 条

[1]

Yang F.J., On-column detection using a fused silica column, Journal of High Resolution Chromatography, 4, (1981)

[2]

Knecht L.A., Guthrie E.J., Jorgenson J.W., Anal. Chem., 56, (1984)

[3]

Guthrie E.J., Jorgenson J.W., Anal. Chem., 56, (1984)

[4]

Sepaniak M.J., Vargo J.D., Kettler C.N., Maskarinec M.P., Anal. Chem., 56, (1984)

[5]

Guthrie E.J., Jorgenson J.W., Dluzneski P.R., J. Chromatogr. Sci., 22, (1984)

[6]

Yang F.J., J. Chromatogr., 236, (1982)

[7]

McGuffin V., Novotny M., Anal. Chem., 55, (1983)

[8]

Jorgenson J.W., Guthrie E.J., J. Chromatogr., 255, (1983)

[9]

Kennedy R.T., Jorgenson J.W., Anal. Chem., 60, (1988)

[10]

Ishii D., Tsuda T., Takeuchi T., J. Chromatogr., 185, (1979)

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