DIRECT EVIDENCE FOR MULTIPLE ENDOTHELIN RECEPTORS

被引:23
作者
SCHVARTZ, I [1 ]
ITTOOP, O [1 ]
HAZUM, E [1 ]
机构
[1] GLAXO RES INST,DIV ENDOCRINOL,5 MOORE DR,RES TRIANGLE PK,NC 27709
关键词
D O I
10.1021/bi00235a028
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Competition binding experiments and peptide mapping techniques were employed in order to directly address the possible existence of endothelin (ET) receptor subtypes in the atria. Competition binding assays for I-125-labeled ET-1 or I-125-labeled ET-3 to bovine atrial membrane preparations suggest the existence of two ET receptor subtypes, one of which binds ET-1 and ET-3 with a similar affinity while the other shows preference for ET-3. However, cross-linking experiments of both peptides to this tissue resulted in the identification of a single 50-kDa protein. To identify directly the existence of multiple ET receptors, peptide mapping of cross-linked I-125-labeled ET-1 or I-125-labeled ET-3 receptors was conducted. Different peptide maps were obtained only under conditions that preferentially label one receptor subtype. These results indicate, for the first time, the existence of two ET receptor subtypes in the atria which differ from each other in both their binding characteristics and primary structure.
引用
收藏
页码:5325 / 5327
页数:3
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