DETECTION OF MEMBRANE-FRAGMENTS IN HEMOGLOBIN-SOLUTIONS

被引：2

作者：

BIESSELS, PTM

BERBERS, GAM

BROEDERS, GCJM

LANDSVATER, R

HUISMAN, J

BLEEKER, WK

BAKKER, JC

机构：

来源：

BIOMATERIALS ARTIFICIAL CELLS AND IMMOBILIZATION BIOTECHNOLOGY | 1992年 / 20卷 / 2-4期

关键词：

D O I：

10.3109/10731199209119665

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Two methods for the detection of membrane components in human stroma-free hemoglobin (SFHb) solutions are described. The first method is a phospholipid assay with a detection limit of 0.5-1 nmol phospholipid/ml SFHb. For the detection of membrane proteins an immunoassay with a monoclonal antibody against glycophorin-alpha was developed (detection limit 0.01% of the original amount). The determination of both glycophorin-alpha and phospholipid yields useful information on the purity of SFHb solutions, as was shown by determination of the purity of two SFHb solutions prepared in different ways.

引用

页码：439 / 442

页数：4

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[4]

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