OUTER-MEMBRANE ANTIGENS OF ORAL TREPONEMA SPECIES

被引：7

作者：

TALL, BD ^{[1
]}

NAUMAN, RK ^{[1
]}

机构：

[1] UNIV MARYLAND, SCH DENT, DEPT MICROBIOL, BALTIMORE, MD 21201 USA

来源：

JOURNAL OF MEDICAL MICROBIOLOGY | 1994年 / 40卷 / 01期

关键词：

D O I：

10.1099/00222615-40-1-62

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Outer membrane (OM) antigens of several oral treponemes were studied. SDS-PAGE revealed a 56-58-kDa protein as a major component of isolated OM vesicles. Immunoblot analysis with eluted antibodies prepared from Treponema denticola ATCC 33520 recognised the 56-58-kDa protein, which was highly conserved in the 17 T. denticola strains tested. The protein was also a component of a T. denticola desoxycholate-extract able, ethanol-soluble antigen (DES-Ag) but was not present in T. pectinovorum or T. vincentii ATCC 35580 and strain N9 whole-cell lysates. Electronmicroscopy of OM vesicles showed typical treponemal ultrastructure. Immunogold labelling of T. denticola ATCC 33520 with T. denticola ATCC 33520-specific eluted antibodies recognised only the OM surface of the cell. These results suggest that the 56-58-kDa antigen comprises surface-orientated epitopes and that this antigen may be specific for T. denticola.

引用

页码：62 / 69

页数：8

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