SYNECHOCYSTIS-6803 PLASTOCYANIN ISOLATED FROM BOTH THE CYANOBACTERIUM AND ESCHERICHIA-COLI TRANSFORMED-CELLS ARE IDENTICAL

被引：34

作者：

HERVAS, M

NAVARRO, F

NAVARRO, JA

CHAVEZ, S

DIAZ, A

FLORENCIO, FJ

DELAROSA, MA

机构：

[1] UNIV SEVILLA,INST BIOQUIM VEGETAL & FOTOSINTESIS,APARTADO 1113,E-41080 SEVILLE,SPAIN

[2] CSIC,E-41080 SEVILLE,SPAIN

来源：

FEBS LETTERS | 1993年 / 319卷 / 03期

关键词：

PETE GENE; PLASTOCYANIN; PROTEIN TRANSLOCATION; SYNECHOCYSTIS;

D O I：

10.1016/0014-5793(93)80558-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Native plastocyanin from Synechocystis 6803 has been isolated and purified to electrophoretic homogeneity. The corresponding gene (petE) has been cloned and expressed in E. coli, thus leading to a protein completely identical to plastocyanin purified from the cyanobacterial cells. The petE gene product is correctly processed in E. coli as deduced from the N-terminal amino acid sequences. These results, along with the identical physicochemical and kinetic properties of the two protein preparations, confirm that expression of petE in E. coli is an adequate tool to address the study of Synechocystis plastocyanin by site-directed mutagenesis.

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页码：257 / 260

页数：4

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