EFFECTS OF GM-CSF DEPRIVATION ON PRECURSORS OF GRANULOCYTES AND MACROPHAGES

被引:63
作者
METCALF, D [1 ]
MERCHAV, S [1 ]
机构
[1] TNO, INST RADIOBIOL, 2280 AA RIJSWIJK, NETHERLANDS
关键词
D O I
10.1002/jcp.1041120315
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Culture of C57BL [mouse neoplastic fibrosarcoma] bone marrow cells in the absence of GM-CSF [granulocyte-macrophage colony-stimulating factor] led to a loss of recoverable granulocyte-macrophage colony-forming cells of 2%/h. The rate of loss of progenitor cells in cultures of CBA fetal liver cells was 5-6%/h. Surviving colony-forming cells exhibited a normal responsiveness to GM-CSF but generated smaller colonies than normal when subsequently stimulated by GM-CSF. Transfer of washed individual day-3 granulocyte-macrophage colony cells to cultures lacking GM-CSF indicated that most cells were unable to survive or proliferate in the absence of GM-CSF. Death of transferred cells was rapid and invariable when the cells were from macrophage-forming colonies. Some cells from 40-70% of granulocyte-forming colonies were able to undergo 1 or 2 divisions in the absence of GM-CSF. This phenomenon was seen most often with cells from colonies where matching colony cells exhibited a higher-than-average proliferative capacity in parallel stimulated cultures. The difficulty that will be encountered in obtaining valid metabolic data from unstimulated populations of granulocyte-macrophage precursor cells is indicated. The ability of some granulocyte precursor cells to exhibit limited proliferation following GM-CSF deprivation suggests that significant amounts of GM-CSF may be bound to or be internalized in some precursor cells, and results in cell division in the absence of GM-CSF from culture medium.
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页码:411 / 418
页数:8
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