INHIBITION OF NFKB DNA-BINDING ACTIVITY BY GLUCOCORTICOIDS IN RAT-BRAIN

被引：59

作者：

UNLAP, T ^{[1
]}

JOPE, RS ^{[1
]}

机构：

[1] UNIV ALABAMA, DEPT PSYCHIAT & BEHAV NEUROBIOL, SPARKS CTR 1057, BIRMINGHAM, AL 35294 USA

来源：

NEUROSCIENCE LETTERS | 1995年 / 198卷 / 01期

关键词：

NFKB; GLUCOCORTICOIDS; SEIZURES; KAINATE; PILOCARPINE; TRANSCRIPTION FACTOR;

D O I：

10.1016/0304-3940(95)11963-W

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The influence of glucocorticoids on the transcription factor NFkB was investigated by using the gel mobility shift assay with nuclear extracts prepared from rat cerebral cortex and hippocampus after a variety of in vivo treatments. Following stimulation with each of three treatments, kainate, pilocarpine, or lithium plus pilocarpine-induced seizures, NFkB DNA binding activity was significantly greater in the cortex and hippocampus from adrenalectomized than from adrenal-intact rats. These results indicate that in rat brain glucocorticoids inhibit NfkB activity in addition to the previously reported inhibition of the transcription factor AP-1 (activator protein 1). Impairment of stimulus-induced transcription factor activity may contribute to the deleterious effects of prolonged elevations of glucocorticoids on neuronal function.

引用

页码：41 / 44

页数：4

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