EXPRESSION OF PHOSPHOGLYCERATE MUTASE MESSENGER-RNA IN DIFFERENTIATING RAT SATELLITE CELL-CULTURES

被引：10

作者：

CASTELLAESCOLA, J

URENA, J

ALTERIO, J

CARRERAS, J

MARTELLY, I

CLIMENT, F

机构：

[1] UNIV BARCELONA, FAC MED, UNITAT BIOQUIM, CASANOVA 143, E-08036 BARCELONA, SPAIN

[2] UNIV PARIS 12, UFR SCI & TECHNOL, MYOGENESE & REGENERAT MUSCULAIRE LAB, F-94000 CRETEIL, FRANCE

来源：

FEBS LETTERS | 1990年 / 268卷 / 01期

关键词：

Cell culture; Isozyme transition; mRNA; Phosphoglycerate mutase;

D O I：

10.1016/0014-5793(90)80963-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Poly (A)+ mRNA was isolated from rat satellite cell cultures and analyzed by Northern blot analyses for mRNA content of phosphoglycerate mutase (PGAM) isozymes. In non-differentiating satellite cells only PGAM-B mRNA was detected, but when cells were differentiated into myotubes, which undergo spontaneous contraction, mRNA for PGAM-M muscle-specific isozyme was also detected. This finding is in perfect concordance with the transition of PGAM isozymes encountered in the same cell cultures, and strongly supports a transcriptional control of PGAM expression throughout myogenesis independently of nerve influence. © 1990.

引用

页码：24 / 26

页数：3

共 22 条

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