ION-PAIR, LIQUID-CHROMATOGRAPHIC ANALYSIS OF TOTAL GOSSYPOL IN CHICKEN LIVER

被引：5

作者：

BOTSOGLOU, NA

SPAIS, AB

机构：

[1] School of Veterinary Medicine, Department of Animal Production, Ichthyology, Ecology and Protection of Environment, Aristotle University, Thessaloniki

来源：

CHROMATOGRAPHIA | 1992年 / 33卷 / 3-4期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; ION-PAIR SYSTEM; GOSSYPOL ANALYSIS; CHICKEN LIVER; PLANT TOXIN RESIDUES;

D O I：

10.1007/BF02275901

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

An ion-pair, liquid chromatographic method for the determination of total gossypol in chicken liver has been developed. It involves hydrolysis of bound gossypol in the presence of ascorbic acid, partitioning into chloroform and isocratic separation on Spherisorb ODS2, 10-mu-m, column with a methanol-water mobile phase containing octane-1-sulfonate. Precision data based on within-day and between-days variation suggested an overall relative standard deviation of 8.1%, while overall recovery was 81.1% +/- 3.

引用

页码：174 / 176

页数：3

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