METABOLITES OF THE FREE-LIVING AMEBA PHREATAMOEBA-BALAMUTHI ANALYZED BY C-13-NMR AND P-31-NMR SPECTROSCOPY - OCCURRENCE OF PHOSPHOINOSITOL DIPHOSPHATES

被引:9
作者
MARTIN, JB
BAKKERGRUNWALD, T
KLEIN, G
机构
[1] CENS,CEA,DEPT BIOL MOLEC & STRUCT,BIOL CELLULAIRE LAB,CNRS,URA 1130,F-38054 GRENOBLE 9,FRANCE
[2] CENS,CEA,DEPT BIOL MOLEC & STRUCT,RESONANCE MAGNET BIOL & MED LAB,GRENOBLE,FRANCE
[3] UNIV OSNABRUCK,MIKROBIOL ABT,W-4500 OSNABRUCK,GERMANY
关键词
D O I
10.1111/j.1550-7408.1995.tb01560.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Phreatamoeba balamuthi is a free-living heterotrophic amoeba that lacks mitochondria. Metabolites of axenically-grown cells were characterized by natural-abundance C-13-NMR and P-31-NMR spectroscopy on acellular perchloric acid extracts. The amoebae were found to contain glycogen and trehalose as storage carbohydrates, together with putrescine and several amino acids, most prominently proline; we propose that proline and trehalose may serve in osmoregulation. Glycerophosphocholine and glycerophosphoethanolamine were present with their phosphomonoester derivatives, phosphocholine and phosphoethanolamine. Along with inorganic phosphate, inorganic pyrophosphate, nucleoside diphosphates, nucleoside triphosphates and NAD, P. balamuthi amoebae also contained unusual phosphoinositol diphosphates in large quantities (0.5 mu mol/g wet cells).
引用
收藏
页码:183 / 191
页数:9
相关论文
共 33 条