TRANSPOSABLE ELEMENTS IN LEPIDOPTERA - HOBO-LIKE TRANSPOSONS IN HELIOTHIS-VIRESCENS AND HELICOVERPA-ZEA

被引：22

作者：

DEVAULT, JD

NARANG, SK

机构：

[1] United States Department of Agriculture, Agricultural Research Service, Fargo, ND 58105

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 203卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.2164

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study, a PCR-based approach was undertaken to determine if members of the hobo-like family of transposable elements were identifiable within the genomes of a number of diverse species, including Heliothis virescens and Helicoverpa tea. The amplified products derived from both H. virescens and H. tea were cloned and characterized. Analysis of the DNA sequence and the single open reading frame found within these fragments clearly demonstrates that these elements are closely related to the hobo transposon from Drosophila melanogaster. In addition, amino acid sequence analysis of the members of this family defines consensus, specific amino acids found within similar regions of all members of this transposon family. (C) 1994 Academic Press, Inc.

引用

页码：169 / 175

页数：7

共 13 条

[1]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[2] IDENTIFICATION OF A FULLY-FUNCTIONAL HOBO TRANSPOSABLE ELEMENT AND ITS USE FOR GERM-LINE TRANSFORMATION OF DROSOPHILA [J].