THE HIGHLY CONSERVED CHINESE-HAMSTER GNAI3 GENE MAPS LESS-THAN 60 KB FROM THE AMPD2 GENE AND LACKS THE INTRONIC U6 SNRNA PRESENT IN ITS HUMAN COUNTERPART

被引：9

作者：

BARON, B ^{[1
]}

FERNANDEZ, MA ^{[1
]}

TOLEDO, F ^{[1
]}

LEROSCOUET, D ^{[1
]}

MAYAU, V ^{[1
]}

MARTIN, N ^{[1
]}

BUTTIN, G ^{[1
]}

DEBATISSE, M ^{[1
]}

机构：

[1] PHARMACIA BIOTECH SA,F-78280 GUYANCOURT,FRANCE

来源：

GENOMICS | 1994年 / 24卷 / 02期

关键词：

D O I：

10.1006/geno.1994.1618

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The identity of a gene coamplified with the adenylate deaminase 2 gene (AMPD2) in coformycin-resistant cells was determined by analysis of its genomic sequence. Sequence comparisons reveal a significant homology with the 3' terminal part of the gene encoding the (alpha i3 subunit of Gi proteins from several species (GNAI3). Identification of the gene was confirmed by Western blot analysis of its products. A precise sequence comparison was performed with the human genomic sequence. It showed that conservation remains important in noncoding exons as well as in introns. However, sequences corresponding to combined U6 snRNA and E protein pseudogene, previously identified inside intron 7 of the human gene, were not found in the Chinese hamster gene. GNAI3 is mapped to a region of conserved linkage between human chromosome 1 (locus 1p13) and mouse chromosome 3 (at 48.4 cM). The Chinese hamster GNAI3 gene maps to chromosome 1 within a 120-kb fragment that also comprises the AMPD2 and GSTM genes. (C) 1994 Academic Press, Inc.

引用

页码：288 / 294

页数：7

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