THE PROTECTIVE EFFECTS OF GLUTATHIONE AGAINST METHYLMERCURY CYTOTOXICITY

被引：53

作者：

KROMIDAS, L

TROMBETTA, LD

JAMALL, IS

机构：

[1] ST JOHNS UNIV,COLL PHARM,TOXICOL PROGRAM,JAMAICA,NY 11439

[2] ST JOHNS UNIV,DEPT BIOL SCI,JAMAICA,NY 11439

[3] CALIF DEPT HLTH SERV,DIV TOX SUBSTANCES CONTROL,SACRAMENTO,CA

[4] UNIV CALIF DAVIS,SACRAMENTO MED CTR,DEPT INTERNAL MED,SACRAMENTO,CA 95817

来源：

TOXICOLOGY LETTERS | 1990年 / 51卷 / 01期

关键词：

Cell culture; Glutathione; Glutathione peroxidase; Methylmercury; Microtubules; Neuroblastoma;

D O I：

10.1016/0378-4274(90)90226-C

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Mouse neuroblastoma cells exposed to 2.5 and 5.0 μM methylmercury for 24 h appeared rounded with the loss of processes. Immunohistochemical staining directed against β-tubulin revealed severe alterations in microtubular architecture. Non-membrane-bound condensation product was visualized ultrastructurally in the treated cells and appeared similar to what was seen histochemically. Reduced and oxidized glutathione levels suggest that methylmercury may manifest its deleterious effects via oxidation of tubulin sulf-hydryls, and by alterations due to peroxidative injury. Cells exposed to methylmercury showed a decrease in glutathione peroxidase activity. Simultaneous administration of 10 mM glutathione with 2.5 and 5.0 μM methylmercury dramatically prevented cell injury. © 1990.

引用

页码：67 / 80

页数：14

共 40 条

[1] BLOCKAGE OF AXOPLASMIC-TRANSPORT AND DEPOLYMERIZATION OF REASSEMBLED MICROTUBULES BY METHYL MERCURY [J].