INFLUENCE OF CULTURAL CONDITIONS ON THE VIRULENCE OF CONIDIA AND BLASTOSPORES OF BEAUVERIA-BASSIANA TO THE GREEN LEAFHOPPER, NEPHOTETTIX-VIRESCENS

被引:49
作者
LANE, BS [1 ]
TRINCI, APJ [1 ]
GILLESPIE, AT [1 ]
机构
[1] AFRC, INST HORT RES, LITTLEHAMPTON BN17 6LP, W SUSSEX, ENGLAND
来源
MYCOLOGICAL RESEARCH | 1991年 / 95卷
关键词
D O I
10.1016/S0953-7562(09)80046-4
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Determinations were made of the median lethal concentration (LC50) and median lethal time (LT50) of conidia and blastospores of B. bassiana against adult Nephotettix virescens (green leafhoppers). There was no significant difference between the LC50 values for blastospores produced in carbon- and nitrogen-limited cultures, but both types of blastospores were significantly less virulent than conidia. The LT50 values of conidia and of blastospores from nitrogen-limited cultures were significantly lower than the value for blastospores produced in carbon-limited culture. The LT50 values of conidia and blastospores increased during storage at 25-degrees-C and were inversely related to spore viability. At all but one concentration tested, nitrogen-limited blastospores 'adhered' to insects more strongly than did carbon-limited blastospores. Fewer spores germinated on insect wings than on agar medium and of the three spore types studied, maximal germination on insect wings was observed with nitrogen-limited blastospores.
引用
收藏
页码:829 / 833
页数:5
相关论文
共 16 条
[1]   NONSPECIFIC FACTORS INVOLVED IN ATTACHMENT OF ENTOMOPATHOGENIC DEUTEROMYCETES TO HOST INSECT CUTICLE [J].
BOUCIAS, DG ;
PENDLAND, JC ;
LATGE, JP .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (07) :1795-1805
[2]  
BOUCIAS DG, 1986, FUNDAMENTAL APPLIED, P432
[3]  
Burges H.D., 1971, Microbial Control of Insects and Mites, P591
[4]   SYNCHRONIZATION OF CELL DIVISION [J].
CAMPBELL, A .
BACTERIOLOGICAL REVIEWS, 1957, 21 (04) :263-272
[5]  
DYCK VA, 1979, BROWN PLANTHOPPER TH, P13
[6]  
Fargues J., 1984, INFECT PROCESSES FUN, P90
[7]  
GRULA EA, 1978, 1ST P JOINT US USSR, P192
[8]   GROWTH AND BLASTOSPORE FORMATION BY PAECILOMYCES-FUMOSOROSEUS, A PATHOGEN OF BROWN PLANTHOPPER (NILAPARVATA-LUGENS) [J].
INCH, JMM ;
HUMPHREYS, AM ;
TRINCI, APJ ;
GILLESPIE, AT .
TRANSACTIONS OF THE BRITISH MYCOLOGICAL SOCIETY, 1986, 87 :215-222
[9]   USE OF THE OPTICAL BRIGHTENER TINOPAL BOPT FOR THE RAPID-DETERMINATION OF CONIDIAL VIABILITIES IN ENTOMOGENOUS DEUTEROMYCETES [J].
JIMENEZ, J ;
GILLESPIE, AT .
MYCOLOGICAL RESEARCH, 1990, 94 :279-283
[10]  
Jimenez J. A., 1989, THESIS U LONDON