CLONING AND EXPRESSION OF A FUNCTIONAL-RAT LIVER D-BETA-HYDROXYBUTYRATE DEHYDROGENASE-BETA-GALACTOSIDASE FUSION PROTEIN IN ESCHERICHIA-COLI

被引：3

作者：

CHURCHILL, S ^{[1
]}

CHURCHILL, P ^{[1
]}

机构：

[1] UNIV ALABAMA,DEPT BIOL,BOX 870344,TUSCALOOSA,AL 35487

来源：

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE | 1991年 / 69卷 / 09期

关键词：

D-BETA-HYDROXYBUTYRATE DEHYDROGENASE; CLONING; EXPRESSION;

D O I：

10.1139/o91-100

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A rat liver bacteriophage-lambda-expression library was probed using polyclonal antibodies raised to purified rat liver D-beta-hydroxybutyrate dehydrogenase (BDH). A clone was selected that contained a 1.2-kb insert. The insert placed in an expression plasmid was utilized to transform Escherichia coli. These cells were shown to possess phosphatidylcholine-dependent BDH activity. Cells transformed with only the plasmid had no detectable BDH activity in the presence of phosphatidylcholine. The expressed activity in E. coli could be inhibited in a dose-dependent manner by BDH antiserum.

引用

页码：670 / 673

页数：4

共 14 条

[1] BAILLY A, 1987, CR SOC BIOL, V181, P506
[2] BOCK HG, 1975, J BIOL CHEM, V250, P5774
[3] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4] CHURCHILL P, 1983, J BIOL CHEM, V258, P208
[5] CLEVELAND DW, 1977, J BIOL CHEM, V252, P1102
[6] FLEISCHER S, 1983, STRUCTURE FUNCTION M, P283
[7] ESTIMATION OF ISOZYMES OF MICROSOMAL CYTOCHROME-P-450 IN RATS, RABBITS, AND HUMANS USING IMMUNOCHEMICAL STAINING COUPLED WITH SODIUM DODECYL-SULFATE POLYACRYLAMIDE-GEL ELECTROPHORESIS
GUENGERICH, FP
WANG, P
DAVIDSON, NK
[J]. BIOCHEMISTRY, 1982, 21 (07) : 1698 - 1706
[8] Kaminsky L S, 1981, Methods Enzymol, V74 Pt C, P262
[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
LAEMMLI, UK
[J]. NATURE, 1970, 227 (5259) : 680 - +
[10] NIELSEN NC, 1973, J BIOL CHEM, V248, P2556

← 1 2 →