A FLEXIBLE, EFFICIENT, CHECKERBOARD IMMUNOBLOT SYSTEM FOR THE DETECTION AND SEMIQUANTITATION OF SPECIFIC ANTINUCLEAR ANTIBODIES

被引：6

作者：

RITCHIE, RF

COLLINS, M

LEDUE, TB

机构：

[1] Foundation for Blood Research, Rheumatic Disease Laboratory, Scarborough, Maine

来源：

JOURNAL OF CLINICAL LABORATORY ANALYSIS | 1992年 / 6卷 / 06期

关键词：

ANTINUCLEAR ANTIBODIES; IMMUNOBLOTTING; REFERENCE MATERIALS;

D O I：

10.1002/jcla.1860060610

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

In this paper an uncomplicated method for the simultaneous detection and semiquantitation of 11 of the 12 commonly studied antinuclear antibodies (ANA) in a single run is described. This new application of checker-board immunoblotting (CBIB) is based upon available technology and employs purified antigens which can be either purchased or produced in-house. CBIB requires no electronic instrument, can be formatted to meet the needs of the user, is rapidly performed, and has acceptable labor and materials costs. Data on the use of the method to examine available reference antisera is presented. CBIB has also proven practical for the clinical study of 18 sera, at two dilutions per membrane, for each set of specific antinuclear antibodies, also at two or more dilutions.

引用

页码：391 / 398

页数：8

共 53 条

[1] ANTI-RO SS-A ANTIBODIES IN THE PATHOPHYSIOLOGY OF CONGENITAL HEART-BLOCK IN NEONATAL LUPUS SYNDROME, AN EXPERIMENTAL-MODEL - INVITRO ELECTROPHYSIOLOGIC AND IMMUNOCYTOCHEMICAL STUDIES [J].