HNF1-ALPHA ACTIVATES THE AMINOPEPTIDASE-N PROMOTER IN INTESTINAL (CACO-2) CELLS

被引：20

作者：

OLSEN, J

LAUSTSEN, L

TROELSEN, J

机构：

[1] Department of Medical Biochemistry and Genetics, Biochemistry Laboratory C, The Panum Institute, Copenhagen N

来源：

FEBS LETTERS | 1994年 / 342卷 / 03期

关键词：

TRANSCRIPTIONAL REGULATION; DIFFERENTIATION; TRANSCRIPTION FACTOR DIMERIZATION; HNF1; AMINOPEPTIDASE N;

D O I：

10.1016/0014-5793(94)80525-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The importance of HNF1 binding proteins for intestinal aminopeptidase N expression was investigated using the Caco-2 cell-line. Aminopeptidase N promoter activity in Caco-2 cells depends on the HNF1 element (positions -85 to -58) and co-transfection with an HNF1 alpha expression vector demonstrates a direct activation of the promoter by HNF1 alpha through this element. Electrophoretic mobility shift assays using nuclear extracts from Caco-2 cells show the presence of high amounts of HNF1 binding proteins irrespective of their state of differentiation.

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页码：325 / 328

页数：4

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