INTERRELATIONSHIPS AMONG ETHANOLAMINE PHOSPHATIDES IN MYELINATING RAT BRAIN

Abstract— —The ethanolamine phosphatide fraction was isolated from rat brain at 17, 19, and 22 days of age. Analysis by gas‐liquid chromatography of the liberated fatty aldehydes and alkyl glyceryl ethers demonstrated a chain length composition quite distinct from that of the fatty acids in the comparable 1(3)‐position of the diacyl phosphatides. [1‐14C]‐Acetate was administered intraperitoneally to 17‐day‐old rats. With the exception of the polyunsaturated fatty acids, isotope was readily incorporated into the individual side chains of the 1‐ and 2‐positions of the glycerol moiety. Time studies revealed no readily discernible precursor‐product relationships among the linkages in question. Therefore, although the long chain precursors for the alkenyl and alkyl ethers may be related by biosynthetic interconversion, the isotope data are suggestive of independent pathways of biosynthesis for the alkenyl ether, alkyl ether, and ester linkages. Copyright © 1969, Wiley Blackwell. All rights reserved