TRANSFORMATION OF RAT CELLS BY THE HYBRID VIRUS AD22+ HEY

被引：9

作者：

GALLOWAY, DA

LUKANIDIN, E

TOPP, WC

SAMBROOK, J

机构：

来源：

JOURNAL OF GENERAL VIROLOGY | 1979年 / 42卷 / FEB期

关键词：

D O I：

10.1099/0022-1317-42-2-339

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A set of four isogenic rat cell lines transformed by Ad22+ HEY have been studied. While all of the cell lines synthesize SV40 T antigen, only one expresses adenovirus 2 T antigen: none expresses SV40 V antigen or adenovirus 2 fibre antigen. Three cell lines contain 1 to 2 virus equivalents of SV40 and adenoviral sequences per diploid quantity of rat cell DNA and the fourth line contains five copies of SV40 and 20 copies of the adenovirus genome. At least three of the cell lines contain DNA sequences from the helper adenovirus 2 in addition to sequences from the Ad2+ HEY genome. The patterns of integrated virus sequences are complex suggesting multiple insertions of both adenovirus and SV40 DNA sequences. SV40 can be rescued from three cell lines by fusion with permissive cells.

引用

页码：339 / 356

页数：18

共 43 条

[1]

BLACK PH, 1967, P NATIONAL ACADEMY S, V54, P374

[2] ARRANGEMENT OF SIMIAN-VIRUS 40 SEQUENCES IN DNA OF TRANSFORMED-CELLS [J].