LACK OF A REQUIREMENT FOR STRICT ROTATIONAL ALIGNMENT AMONG TRANSCRIPTION FACTOR BINDING-SITES IN YEAST

被引：3

作者：

INOKUCHI, K ^{[1
]}

NAKAYAMA, A ^{[1
]}

机构：

[1] MITSUBISHI KASEI INST LIFE SCI,MOLEC GENET LAB,MACHIDA,TOKYO 194,JAPAN

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 11期

关键词：

D O I：

10.1093/nar/19.11.3099

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The MF-alpha-1 gene of Saccharomyces cerevisiae is an alpha-specific gene whose expression is regulated by two upstream activation sites (UAS(MF-alpha-1)S), which are the binding sites for activator proteins, MAT-alpha-1 and MCM1. UAS(MF-alpha-1) elements act in a synergistic fashion; lack of either element causes reduced expression levels that are 6- to 45-fold less than that of intact promoter in alpha-cells. We investigated the effect of manipulating rotational alignments among transcription factor binding sites of MF-alpha-1 on the expression of the gene. The expression level of MF-alpha-1 decreased with the spacing between the two UAS(MF-alpha-1)S and no synergism was observed when the distance of the two elements was longer than 90 base pairs. No strict rotational alignment was required for their synergistic action. We also show that the spacing between UAS(MF-alpha-1) elements and TFIID binding site, the TATA box, had little, if any, effect on MF-alpha-1 expression. We chose a sufficient number of positions for UAS(MF-alpha-1)S to ensure that, in several of these positions, MAT-alpha-1 and MCM1 were on the opposite side of the DNA helix with respect to the TATA box.

引用

页码：3099 / 3103

页数：5

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