SIMULTANEOUS DETERMINATION OF 3-HYDROXYANTHRANILIC AND CINNABARINIC ACID BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH PHOTOMETRIC OR ELECTROCHEMICAL DETECTION

被引：11

作者：

CHRISTEN, S ^{[1
]}

STOCKER, R ^{[1
]}

机构：

[1] HEART RES INST,BIOCHEM GRP,145 MISSENDEN RD,SYDNEY,NSW 2050,AUSTRALIA

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 200卷 / 02期

关键词：

D O I：

10.1016/0003-2697(92)90465-J

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A convenient and rapid method for the simultaneous determination by HPLC of 3-hydroxyanthranilic acid and the dimer derived by its oxidation, cinnabarinic acid, is described. Buffers or biological samples containing these two Trp metabolites were acidified to pH 2.0 and extracted with ethyl acetate with recoveries of 96.5 ± 0.5 and 93.4 ± 3.7% for 3-hydroxyanthranilic and cinnabarinic acid, respectively. The two compounds were separated on a reversed-phase (C18) column combined with ion-pair chromatography and detected photometrically or electrochemically. The method was applied successfully to biological systems in which formation of either 3-hydroxyanthranilic or cinnabarinic acid had been described previously. Thus, interferon-γ-treated human peripheral blood mononuclear cells formed and released significant amounts of 3-hydroxyanthranilic acid into the culture medium and mouse liver nuclear fraction possessed high "cinnabarinic acid synthase" activity. In contrast, addition of 3-hydroxyanthranilic acid to human erythrocytes resulted in only marginal formation of cinnabarinic acid. We conclude that the method described is specific, sensitive, and suitable for the detection of the two Trp metabolites in biological systems. © 1992.

引用

页码：273 / 279

页数：7

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