ASSAY METHODOLOGY FOR PREDNISOLONE, PREDNISOLONE ACETATE AND PREDNISOLONE SODIUM-PHOSPHATE IN RABBIT AQUEOUS-HUMOR AND OCULAR PHYSIOLOGICAL SOLUTIONS

被引：11

作者：

MUSSON, DG

BIDGOOD, AM

OLEJNIK, O

机构：

[1] IOLAB Corporation (Johnson and Johnson Company), Claremont, CA 91711

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 565卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80373-K

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

Prednisolone, prednisolone acetate and prednisolone sodium phosphate are glucocorticoids used for ocular, anti-inflammatory therapy. A reversed-phase high-performance liquid chromatographic assay using ultraviolet detection has been developed that affords baseline resolution of the above analytes in balanced salt solutions and rabbit aqueous humor. The drugs can be quantified at 0.025-0.05-mu-g/ml in the above matrices; 6-alpha-methylprednisolone is used as the internal standard. Both esters of prednisolone are vulnerable to chemical and enzymatic hydrolysis giving prednisolone. Analysis of aqueous humor samples shows prednisolone acetate penetrating/metabolizing primarily to prednisolone; prednisolone sodium phosphate penetrates the cornea giving the ester and alcohol.

引用

页码：89 / 102

页数：14

共 18 条

[1]

COMPARISON OF RADIOIMMUNOASSAY AND THIN-LAYER CHROMATOGRAPHIC ASSAY-METHODS FOR ESTIMATION OF PLASMA PREDNISOLONE CONCENTRATIONS [J].