THRESHOLD FLUENCES FOR PRODUCTION OF POSITIVE AND NEGATIVE-IONS IN MATRIX-ASSISTED LASER DESORPTION IONIZATION USING LIQUID AND SOLID MATRICES

被引：27

作者：

YAU, PY

CHAN, TWD

CULLIS, PG

COLBURN, AW

DERRICK, PJ

机构：

[1] UNIV WARWICK,DEPT CHEM,COVENTRY CV4 7AL,W MIDLANDS,ENGLAND

[2] PHILLIP INST TECHNOL,DEPT APPL CHEM,BUNDOORA,VIC,AUSTRALIA

来源：

CHEMICAL PHYSICS LETTERS | 1993年 / 202卷 / 1-2期

关键词：

D O I：

10.1016/0009-2614(93)85356-S

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Dependences of positive and negative analyte-ion and matrix-ion signals upon pulse energy (at 266 nm) have been measured in matrix-assisted laser desorption/ionization (MALDI), for a liquid matrix (3-nitrobenzyl alcohol) and for a solid matrix (sinapinic acid). The analyte was the protein bovine insulin. The results suggest that the threshold fluences for the formation of positive and of negative ions are the same as each other, for a given matrix. The threshold fluences are lower in the case of the liquid matrix as compared to the solid matrix.

引用

收藏

页码：93 / 100

页数：8

相关论文

共 16 条

[1] VELOCITY DISTRIBUTIONS OF INTACT HIGH MASS POLYPEPTIDE MOLECULE IONS PRODUCED BY MATRIX ASSISTED LASER DESORPTION [J].

BEAVIS, RC ;

CHAIT, BT .

CHEMICAL PHYSICS LETTERS, 1991, 181 (05) :479-484

[2] PHENOMENOLOGICAL MODELS FOR MATRIX-ASSISTED LASER DESORPTION ION YIELDS NEAR THE THRESHOLD FLUENCE [J].

BEAVIS, RC .

ORGANIC MASS SPECTROMETRY, 1992, 27 (08) :864-868

[3] SUPPRESSION OF MATRIX IONS IN ULTRAVIOLET-LASER DESORPTION - SCANNING ELECTRON-MICROSCOPY AND RAMAN-SPECTROSCOPY OF THE SOLID SAMPLES [J].

CHAN, TWD ;

COLBURN, AW ;

DERRICK, PJ ;

GARDINER, DJ ;

BOWDEN, M .

ORGANIC MASS SPECTROMETRY, 1992, 27 (03) :188-194

[4] MATRIX-ASSISTED UV LASER DESORPTION - SUPPRESSION OF THE MATRIX PEAKS [J].

CHAN, TWD ;

COLBURN, AW ;

DERRICK, PJ .

ORGANIC MASS SPECTROMETRY, 1991, 26 (04) :342-344

[5]

CHAN TWD, 1992, ORG MASS SPECTROM, V27, P55

[6] MATRIX-ASSISTED LASER DESORPTION WITH ULTRA-SHORT LASER-PULSES [J].

DEMIREV, P ;

WESTMAN, A ;

REIMANN, CT ;

HAKANSSON, P ;

BAROFSKY, D ;

SUNDQVIST, BUR ;

CHENG, YD ;

SEIBT, W ;

SIEGBAHN, K .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1992, 6 (03) :187-191

[7]

ENS W, 1991, RAPID COMMUN MASS SP, V5, P17

[8] ULTRAVIOLET-LASER DESORPTION IONIZATION MASS-SPECTROMETRY OF FEMTOMOLAR AMOUNTS OF LARGE PROTEINS [J].

KARAS, M ;

INGENDOH, A ;

BAHR, U ;

HILLENKAMP, F .

BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, 1989, 18 (09) :841-843

[9] LASER DESORPTION IONIZATION MASS-SPECTROMETRY OF PROTEINS OF MASS 100 000 TO 250 000 DALTON [J].

KARAS, M ;

BAHR, U ;

INGENDOH, A ;

HILLENKAMP, F .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1989, 28 (06) :760-761

[10] LASER DESORPTION IONIZATION OF PROTEINS WITH MOLECULAR MASSES EXCEEDING 10000 DALTONS [J].

KARAS, M ;

HILLENKAMP, F .

ANALYTICAL CHEMISTRY, 1988, 60 (20) :2299-2301