A REGION OF THE BROAD-HOST-RANGE PLASMID RK2 CAUSES STABLE IN PLANTA INHERITANCE OF PLASMIDS IN RHIZOBIUM-MELILOTI CELLS ISOLATED FROM ALFALFA ROOT-NODULES

被引：61

作者：

WEINSTEIN, M

ROBERTS, RC

HELINSKI, DR

机构：

[1] UNIV CALIF SAN DIEGO,DEPT BIOL,LA JOLLA,CA 92093

[2] UNIV CALIF SAN DIEGO,CTR MOLEC GENET,LA JOLLA,CA 92093

来源：

JOURNAL OF BACTERIOLOGY | 1992年 / 174卷 / 22期

关键词：

D O I：

10.1128/jb.174.22.7486-7489.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We demonstrate for the first time that the broad-host-range stabilization loci from plasmid RK2 cause total retention of plasmids in cells of Rhizobium meliloti during symbiosis with alfalfa. Two derivatives of plasmid RK2, pRK290 and a 7.3-kb mini-RK2 plasmid, were stabilized in R. meliloti cells isolated from root nodules by the insertion of a 3.2-kb DNA fragment or a smaller 0.8-kb DNA fragment derived from the RK2 stabilization region.

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页码：7486 / 7489

页数：4

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