GENE-TRANSFER AND EXPRESSION IN PROGENY AFTER INTRAVENOUS DNA INJECTION INTO PREGNANT MICE

Several methods that enable foreign genes to be transferred directly into germ cells and adult animals have been developed(1-4), which have stimulated great interest in manipulating genes in vivo. However, there have been no methods available for introducing genes into fetuses. We report here that a single intravenous injection of expression plasmid:lipopolyamine complexes into pregnant mice resulted in successful gene transfer into the embryos. The transgenes thus introduced were expressed in the fetuses and newborn progeny. This simple and new method of gene transfer into embryos will facilitate rapid analysis of transgene effects in the fetuses and will be useful for studying gene-deficient animal models to gain transgene functions at desired stages of embryogenesis.