PHENOTYPIC CHARACTERIZATION OF NORMAL AND CML CD34-POSITIVE CELLS - ONLY THE MOST PRIMITIVE CML PROGENITORS INCLUDE PH-NEG CELLS

被引：8

作者：

DEFABRITIIS, P ^{[1
]}

DOWDING, C ^{[1
]}

BUNGEY, J ^{[1
]}

CHASE, A ^{[1
]}

ANGUS, G ^{[1
]}

SZYDLO, R ^{[1
]}

GOLDMAN, JM ^{[1
]}

机构：

[1] HAMMERSMITH HOSP,ROYAL POSTGRAD MED SCH,LRF LEUKAEMIA UNIT,LONDON W12 ONN,ENGLAND

来源：

LEUKEMIA & LYMPHOMA | 1993年 / 11卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

PHENOTYPE; NORMAL CD34 CELLS; CML; PROGENITORS; CML PROGENITORS; PH-NEG CELLS;

D O I：

10.3109/10428199309054730

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We studied the sequence.of acquisition of CD33, CD38 and HLA-DR antigens on CD34+ cells from marrow and blood of Ph-chromosome positive CML patients and normal marrow. We examined the Ph status of the various CML cell populations. The mean proportions of normal and CML CD34+ cells expressing CD33 and CD38 were not significantly different. However, a significantly greater proportion of CML CD34+ cells expressed HLA-DR antigens compared with normal CD34+ cells and the level of HLA-DR expression per CML cell was abnormally high. When the sequence of acquisition of these antigens on normal and CML CD34+ cells was evaluated using 3-colour fluorescence analysis, the results suggested that HLA-DR was expressed earlier than CD38 or CD33 and these findings were confirmed by following the acquisition of CD38 and CD34+/DR+/CD38-subpopulation during liquid culture. We performed cytogenetic studies on CD34+ subpopulations in 6 cases. In 4 cases there were some Ph-negative metaphases detectable in the CD34+/DR-subpopulation (range 12.5 to 60%). In the CD34+/DR+ fractions, however, all 6 patients had only Ph-positive metaphases and only 1/5 patients had detectable Ph-negative metaphases in the CD34+/CD38-subpopulation. We conclude that expression of HLA-DR antigens may precede the expression of CD38 on CD34+ cells during normal stem cell differentiation. In CML DR may be expressed aberrantly and Ph-negative cells are found predominantly in the DR negative subpopulation.

引用

页码：51 / 61

页数：11

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