OPTIMIZATION OF QUANTITATIVE CULTURE ASSAY FOR HUMAN-IMMUNODEFICIENCY-VIRUS FROM PLASMA

被引：12

作者：

LATHEY, JL

FISCUS, SA

RASHEED, S

KAPPES, JC

GRIFFITH, BP

ELBEIK, T

SPECTOR, SA

REICHELDERFER, PS

机构：

[1] UNIV N CAROLINA, DEPT MICROBIOL & IMMUNOL, CHAPEL HILL, NC 27599 USA

[2] UNIV SO CALIF, VIRAL ONCOL & AIDS RES LAB, LOS ANGELES, CA 90032 USA

[3] UNIV ALABAMA, DEPT MED, BIRMINGHAM, AL 35294 USA

[4] YALE UNIV, SCH MED, DEPT LAB MED, NEW HAVEN, CT 06510 USA

[5] UNIV CALIF SAN FRANCISCO, DEPT MED, SAN FRANCISCO, CA 94143 USA

[6] NIAID, DIV AIDS, BETHESDA, MD 20892 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1994年 / 32卷 / 12期

关键词：

D O I：

10.1128/JCM.32.12.3064-3067.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Experimental conditions essential to a simple, reproducible, quantitative human immunodeficiency virus plasma assay were determined. Five parameters were evaluated: length of culture, reproducibility, assay dilution schema, washing procedures, and anticoagulant usage. The recommended quantitative plasma assay utilizes undiluted citrated plasma cultured with peripheral blood mononuclear cells for 14 days with fivefold dilutions and a medium change on day 1 with no washing.

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收藏

页码：3064 / 3067

页数：4

相关论文

共 11 条

[1] CELL-FREE PLASMA HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 TITER ASSESSED BY CULTURE AND IMMUNOCAPTURE-REVERSE TRANSCRIPTION-POLYMERASE CHAIN-REACTION [J].

COOMBS, RW ;

HENRARD, DR ;

MEHAFFEY, WF ;

GIBSON, J ;

EGGERT, E ;

QUINN, TC ;

PHILLIPS, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (08) :1980-1986

[2]

COOMBS RW, 1989, NEW ENGL J MED, V321, P1621

[3]

DEWAR RL, 1992, J ACQ IMMUN DEF SYND, V5, P822

[4] USE OF VIROLOGICAL ASSAYS FOR DETECTION OF HUMAN-IMMUNODEFICIENCY-VIRUS IN CLINICAL-TRIALS - RECOMMENDATIONS OF THE AIDS CLINICAL-TRIALS GROUP VIROLOGY COMMITTEE [J].

HAMMER, S ;

CRUMPACKER, C ;

DAQUILA, R ;

JACKSON, B ;

LATHEY, J ;

LIVNAT, D ;

REICHELDERFER, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (10) :2557-2564

[5] QUANTITATION OF HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 IN THE BLOOD OF INFECTED PERSONS [J].

HO, DD ;

MOUDGIL, T ;

ALAM, M .

NEW ENGLAND JOURNAL OF MEDICINE, 1989, 321 (24) :1621-1625

[6] INHIBITION OF HUMAN-IMMUNODEFICIENCY-VIRUS GENE AMPLIFICATION BY HEPARIN [J].

HOLODNIY, M ;

KIM, S ;

KATZENSTEIN, D ;

KONRAD, M ;

GROVES, E ;

MERIGAN, TC .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (04) :676-679

[7]

OROSZLAN S, 1990, CURR TOP MICROBIOL, V157, P153

[8] DETECTION OF PLASMA VIREMIA IN HUMAN IMMUNODEFICIENCY VIRUS-INFECTED INDIVIDUALS AT ALL CLINICAL STAGES [J].

PAN, LZ ;

WERNER, A ;

LEVY, JA .

JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (02) :283-288

[9] HIGH-LEVEL VIREMIA IN ADULTS AND CHILDREN INFECTED WITH HUMAN-IMMUNODEFICIENCY-VIRUS - RELATION TO DISEASE STAGE AND CD4+ LYMPHOCYTE LEVELS [J].

SAAG, MS ;

CRAIN, MJ ;

DECKER, WD ;

CAMPBELLHILL, S ;

ROBINSON, S ;

BROWN, WE ;

LEUTHER, M ;

WHITLEY, RJ ;

HAHN, BH ;

SHAW, GM .

JOURNAL OF INFECTIOUS DISEASES, 1991, 164 (01) :72-80

[10] EFFECT OF DIDANOSINE ON HUMAN-IMMUNODEFICIENCY-VIRUS VIREMIA AND ANTIGENEMIA IN PATIENTS WITH ADVANCED DISEASE - CORRELATION WITH CLINICAL-RESPONSE [J].

SHEPP, DH ;

ASHRAF, A .

JOURNAL OF INFECTIOUS DISEASES, 1993, 167 (01) :30-35