PURIFICATION AND CHARACTERIZATION OF AN ENDOTHELIAL CELL-VIABILITY MAINTAINING FACTOR FROM FETAL BOVINE SERUM

被引：18

作者：

CAI, G ^{[1
]}

SATOH, T ^{[1
]}

HOSHI, H ^{[1
]}

机构：

[1] RES INST FUNCT PEPTIDES, YAMAGATA 990, JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1995年 / 1269卷 / 01期

关键词：

ATHEROSCLEROSIS; APOLIPOPROTEIN H; SURVIVAL FACTOR; ENDOTHELIAL CELL; SERUM-FREE MEDIUM;

D O I：

10.1016/0167-4889(95)00091-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Serum is an essential requirement for the growth and long-term survival of human endothelial cells, even in the presence of such defined elements such as polypeptide growth factors and hormones. A polypeptide from fetal bovine serum was isolated and characterized on the basis of long-term survival of human endothelial cells in serum-free culture. The endothelial cell viability maintaining factor has been purified to homogeneity by a combination of polyethylene glycol precipitation, hydroxylapatite, gel permeation and reverse-phase high performance liquid chromatography. The final purified endothelial cell viability maintaining factor has a molecular weight of 65 000 (reduced) and has been identified as bovine apolipoprotein H by amino-terminal amino acid sequence analysis and Western blot analysis. Endothelial cell viability maintaining factor improved a long-term viability of human endothelial cells at maximal concentrations of 2.5-5 mu g protein/ml in serum-free medium.

引用

页码：13 / 18

页数：6

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