RECONSTITUTION INTO LIPOSOMES OF GLUCOSE ACTIVE-TRANSPORT FROM THE RABBIT RENAL PROXIMAL TUBULE - CHARACTERISTICS OF THE SYSTEM

被引：50

作者：

FAIRCLOUGH, P

MALATHI, P

PREISER, H

CRANE, RK

机构：

[1] College of Medicine and Dentistry, New Jersey Rutgers Medical School, Department of Physiology and Biophysics, Piscataway

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 553卷 / 02期

基金：

美国国家科学基金会;

关键词：

(Rabbit kidney); Glucose transport; Liposome; Reconstitution;

D O I：

10.1016/0005-2736(79)90233-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper describes the characteristics of Na+-dependent d-glucose transport into liposomes made from soybean phospholipids into which have been reconstituted detergent-solubilized components from the rabbit renal proximal tubular brush border membrane. Conditions for optimal and quantitative reconstitution of glucose carriers are defined. Na+-dependent d-glucose uptake occurs via a saturable system with a Km of 0.125-0.135 mM, is responsive to the volume of the internal liposomal space, and shows 'overshoot' as seen in natural membranes. The rate of Na+-dependent d-glucose uptake and the magnitude of the 'overshoot' are proportional to the concentration of protein used in reconstitution. © 1979.

引用

页码：295 / 306

页数：12

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