AMPLIFICATION AND DIFFERENTIATION OF THE DNA OF AN ABORTIGENIC (TYPE-1) AND A RESPIRATORY (TYPE-4) STRAIN OF EQUINE HERPESVIRUS BY THE POLYMERASE CHAIN-REACTION

被引：30

作者：

OKEEFE, JS

MURRAY, A

WILKS, CR

MORIARTY, KM

机构：

[1] Department of Veterinary Pathology and Public Health, Massey University, Palmerston North

来源：

RESEARCH IN VETERINARY SCIENCE | 1991年 / 50卷 / 03期

关键词：

D O I：

10.1016/0034-5288(91)90137-D

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Unpurified DNA derived from cultures of equine fetal kidney cells infected with either equine herpesvirus type 1 or equine herpesvirus type 4 was amplified by the polymerase chain reaction using one pair of oligonucleotide primers. Restriction endonuclease digestion of the amplified segments with PvuII, followed by electrophoresis, revealed restriction fragment length polymorphisms which enabled the two virus types to be differentiated.

引用

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页码：349 / 351

页数：3

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