TEMPERATURE EFFECTS ON THE AGGREGATION STATE AND ACTIVITY OF AMPHOTERICIN-B

被引：31

作者：

LAMBING, HE ^{[1
]}

WOLF, BD ^{[1
]}

HARTSEL, SC ^{[1
]}

机构：

[1] UNIV WISCONSIN,DEPT CHEM,EAU CLAIRE,WI 54702

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1152卷 / 01期

基金：

美国国家科学基金会;

关键词：

AMPHOTERICIN-B; NYSTATIN; PYRANINE; ION CURRENT; DRUG DELIVERY; LIPOSOME;

D O I：

10.1016/0005-2736(93)90246-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Amphotericin B (AmB) in aqueous solution becomes less aggregated as temperature is increased as measured by CD spectroscopy. Concomitantly, AmB's ability to induce K+ leakage from cholesterol-containing large unilamellar vesicles (LUV) decreases 4-fold per 10-degrees-C increase. In contrast, ergosterol-containing vesicles show no clear trend in their temperature sensitivity to AmB action. These data strongly support a model of AmB action where drug aggregates and not monomers are the major channel-inducing species against cholesterol- but not ergosterol-containing membranes.

引用

页码：185 / 188

页数：4

共 20 条

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BENZ S K, 1991, Biophysical Journal, V59, p323A

[2] HOW DO THE POLYENE MACROLIDE ANTIBIOTICS AFFECT THE CELLULAR MEMBRANE-PROPERTIES [J].