INTERSPECIFIC SOMATIC HYBRIDIZATION BETWEEN SOLANUM-TUBEROSUM L AND S-BULBOCASTANUM DUN AS A MEANS OF TRANSFERRING NEMATODE RESISTANCE

被引：64

作者：

AUSTIN, S

POHLMAN, JD

BROWN, CR

MOJTAHEDI, H

SANTO, GS

DOUCHES, DS

HELGESON, JP

机构：

[1] WASHINGTON STATE UNIV,IAREC,PROSSER,WA 99350

[2] MSU,DEPT CROP & SOIL SCI,E LANSING,MI 48824

[3] USDA ARS,DEPT PLANT PATHOL,MADISON,WI 53706

[4] USDA ARS,PWR,WASHINGTON,DC 20250

来源：

AMERICAN POTATO JOURNAL | 1993年 / 70卷 / 06期

关键词：

PROTOPLAST FUSION; POTATO; PEST RESISTANCE; BREEDING; MELOIDOGYNE-CHITWOODI;

D O I：

10.1007/BF02849067

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Interspecific somatic hybrids were produced between tetraploid Solanum tuberosum and a nematode-resistant accession of the diploid species Solanum bulbocastanum by protoplast fusion. Hybrid cells were selected using dual fluorescent labeling of protoplast preparations prior to fusion. Hybridity of regenerated plants was confirmed with a combination of morphological assessment, chromosome counting and isozyme analysis. Somatic hybrids had the same level of resistance to infection by race 1 of the nematode Meloidogyne chitwoodi as the S. bulbocastanum parent used in the fusion. Some of the somatic hybrids were fertile as females when crossed with tetraploid S. tuberosum breeding lines. Thus, these hybrids can be used in a potato improvement program to incorporate a valuable pest resistance.

引用

页码：485 / 495

页数：11

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