GENE ENCODING A NOVEL EXTRACELLULAR METALLOPROTEASE IN BACILLUS-SUBTILIS

被引：56

作者：

SLOMA, A

RUDOLPH, CF

RUFO, GA

SULLIVAN, BJ

THERIAULT, KA

ALLY, D

PERO, J

机构：

[1] BioTechnica International Inc., Cambridge, MA 02140

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 02期

关键词：

D O I：

10.1128/jb.172.2.1024-1029.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The gene for a novel extracellular metalloprotease was cloned, and its nucleotide sequence was determined. The gene (mpr) encodes a primary product of 313 amino acids that has little similarity to other known Bacillus proteases. The amino acid sequences of the mature protease was preceded by a signal sequence of approximately 34 amino acids and a pro sequence of 58 amino acids. -ttl

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页码：1024 / 1029

页数：6

相关论文

共 30 条

[1] REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS [J].

ANAGNOSTOPOULOS, C ;

SPIZIZEN, J .

JOURNAL OF BACTERIOLOGY, 1961, 81 (05) :741-&

[2] DEOXYNUCLEOSIDE PHOSPHORAMIDITES - A NEW CLASS OF KEY INTERMEDIATES FOR DEOXYPOLYNUCLEOTIDE SYNTHESIS [J].

BEAUCAGE, SL ;

CARUTHERS, MH .

TETRAHEDRON LETTERS, 1981, 22 (20) :1859-1862

[3]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[4] CONSTRUCTION OF A KIT OF REFERENCE STRAINS FOR RAPID GENETIC-MAPPING IN BACILLUS-SUBTILIS-168 [J].

DEDONDER, RA ;

LEPESANT, JA ;

LEPESANTKEJZLAROVA, J ;

BILLAULT, A ;

STEINMETZ, M ;

KUNST, F .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1977, 33 (04) :989-993

[5] FATE OF TRANSFORMING DNA FOLLOWING UPTAKE BY COMPETENT BACILLUS-SUBTILIS [J].

DUBNAU, D ;

DAVIDOFF.R .

JOURNAL OF MOLECULAR BIOLOGY, 1971, 56 (02) :209-&

[6] COLONY HYBRIDIZATION - METHOD FOR ISOLATION OF CLONED DNAS THAT CONTAIN A SPECIFIC GENE [J].

GRUNSTEIN, M ;

HOGNESS, DS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (10) :3961-3965

[7] CHARACTERIZATION OF CHIMERIC PLASMID CLONING VEHICLES IN BACILLUS-SUBTILIS [J].

GRYCZAN, T ;

SHIVAKUMAR, AG ;

DUBNAU, D .

JOURNAL OF BACTERIOLOGY, 1980, 141 (01) :246-253

[8] CHARACTERIZATION OF STAPHYLOCOCCUS-AUREUS PLASMIDS INTRODUCED BY TRANSFORMATION INTO BACILLUS-SUBTILIS [J].

GRYCZAN, TJ ;

CONTENTE, S ;

DUBNAU, D .

JOURNAL OF BACTERIOLOGY, 1978, 134 (01) :318-329

[9] HYPERPROTEASE-PRODUCING MUTANTS OF BACILLUS-SUBTILIS [J].

HIGERD, TB ;

HOCH, JA ;

SPIZIZEN, J .

JOURNAL OF BACTERIOLOGY, 1972, 112 (02) :1026-&

[10] TRANSFORMATION AND TRANSDUCTION IN RECOMBINATION-DEFECTIVE MUTANTS OF BACILLUS SUBTILIS [J].

HOCH, JA ;

BARAT, M ;

ANAGNOST.C .

JOURNAL OF BACTERIOLOGY, 1967, 93 (06) :1925-&