MOLECULAR-CLONING OF XENOPUS FIBRILLARIN, A CONSERVED U3 SMALL NUCLEAR RIBONUCLEOPROTEIN RECOGNIZED BY ANTISERA FROM HUMANS WITH AUTOIMMUNE-DISEASE

被引：122

作者：

LAPEYRE, B

MARIOTTINI, P

MATHIEU, C

FERRER, P

AMALDI, F

AMALRIC, F

CAIZERGUESFERRER, M

机构：

[1] CNRS,CTR RECH BIOCHIM & GENET CELLULAIRES,118 ROUTE NARBONNE,F-31062 TOULOUSE,FRANCE

[2] UNIV ROMA TOR VERGATA 2,DIPARTIMENTO BIOL,I-00173 ROME,ITALY

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 01期

关键词：

D O I：

10.1128/MCB.10.1.430

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Autoantibodies against U3 small nuclear ribonucleoprotein are associated with scleroderma autoimmune disease. They were shown to react with fibrillarin, a 34- to 36-kilodalton protein that has been detected in all eukaryotes tested from humans to yeasts. We isolated a 1.6-kilobase cDNA clone encoding fibrillarin from a Xenopus laevis cDNA library. The protein contains a 79-residue-long Gly-Arg-rich domain in its N-terminal region and a putative RNA-binding domain with ribonucleoprotein consensus sequence in its central portion. This is the first report of cloning of fibrillarin, and the deduced protein sequence is in agreement with the involvement of the protein in a ribonucleoprotein particle.

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页码：430 / 434

页数：5

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