PEROXIDATION IN POSITION C-6 OF PROGESTERONE-3-ETHANOLIMINE IS INCREASED BY THE PRESENCE OF ENZYMES GENERATING OXYGEN RADICALS

被引：3

作者：

ERIKSSON, CG

ENEROTH, P

机构：

[1] Department of Clinical Chemistry and Blood Coagulation, Karolinska Hospital, Stockholm

[2] Unit for Applied Biochemistry, The Clinical Research Centre, Huddinge Hospital, Huddinge

来源：

STEROIDS | 1990年 / 55卷 / 08期

关键词：

STEROIDS; PEROXIDATION; PROGESTERONE-3-IMINE; ANDROSTENEDIONE-3-IMINE;

D O I：

10.1016/0039-128X(90)90061-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The generation of 6-oxygenated (6-beta-hydroxy, 6-beta-hydroperoxy, and 6-oxo) progesterone derivatives during the hydrolysis of progesterone-3-ethanolimine has been shown to be increased in the presence of xanthine/xanthine oxidase. The combination of xanthine/xanthine oxidase with other enzymes and/or reagents that catalyze transformation (or formation) of oxygen radicals suggested that the most likely oxygen species participating in the 6-oxygenation was the protonated acid of the superoxide anion, i.e., the hydroperoxy radical. The suggestion was further supported by experiments with oxygen scavengers. However, the data presented do not rule out a radical propagation reaction since the steroid compound used may be more reactive than the scavengers tested. A stimulation of 6-oxygenation of progesterone-3-ethanolimine by NADPH-supplemented rat liver microsomes was found. This reaction was inhibited by the only oxygen scavenger (reduced glutathione) found to be effective in the xanthine/xanthine oxidase experiments. The similarities between the two oxygenation systems may implicate a mechanism for 6-beta-hydroperoxidation of 3-oxo-4-ene steroids in rat liver microsomes.

引用

页码：366 / 372

页数：7

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