EFFECT OF POLYETHYLENE-GLYCOL ON THE SYNTHESIS OF OLIGOPEPTIDE BY PAPAIN IN AN ORGANIC MEDIUM

被引：6

作者：

HIRANO, Y

TERAI, T

GOTO, K

NAKAJIMA, A

机构：

[1] Department of Applied Chemistry, Osaka Institute of Technology, Asahi-ku, Osaka, 535

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1991年 / 55卷 / 10期

关键词：

D O I：

10.1080/00021369.1991.10870989

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The enzymic peptide synthesis in an organic medium was investigated by using papain in the presence of PEG. The added PEG enhanced the enzyme activity of papain for synthesis of the peptide, Boc-Gly-Asp(OBzl)OBzl. The activity papain in the PEG-added system was higher than that in a PEG-free system.

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页码：2461 / 2465

页数：5

相关论文

共 16 条

[1] ENZYMES IN NON-AQUEOUS SOLVENTS [J].

BUTLER, LG .

ENZYME AND MICROBIAL TECHNOLOGY, 1979, 1 (04) :253-259

[2] ENZYMATIC CATALYSIS IN MONOPHASIC ORGANIC-SOLVENTS [J].

DORDICK, JS .

ENZYME AND MICROBIAL TECHNOLOGY, 1989, 11 (04) :194-211

[3] APPLICATION OF POLYETHYLENE GLYCOL-MODIFIED ENZYMES IN BIOTECHNOLOGICAL PROCESSES - ORGANIC SOLVENT-SOLUBLE ENZYMES [J].

INADA, Y ;

TAKAHASHI, K ;

YOSHIMOTO, T ;

AJIMA, A ;

MATSUSHIMA, A ;

SAITO, Y .

TRENDS IN BIOTECHNOLOGY, 1986, 4 (07) :190-194

[4] ENGINEERING PHYSICOCHEMICAL AND BIOLOGICAL PROPERTIES OF PROTEINS BY CHEMICAL MODIFICATION [J].

INADA, Y ;

YOSHIMOTO, T ;

MATSUSHIMA, A ;

SAITO, Y .

TRENDS IN BIOTECHNOLOGY, 1986, 4 (03) :68-73

[5]

INADA Y, 1987, PROTEIN HYBRIDES, P1

[6]

KLIBANOV AM, 1986, CHEMTECH, P354

[7] CHYMOTRYPSIN MODIFIED WITH POLYETHYLENE-GLYCOL CATALYZES PEPTIDE-SYNTHESIS REACTION IN BENZENE [J].

MATSUSHIMA, A ;

OKADA, M ;

INADA, Y .

FEBS LETTERS, 1984, 178 (02) :275-277

[8] DESIGN OF PROTEINASE-CATALYZED SYNTHESIS OF OLIGOPEPTIDES IN AN AQUEOUS-ORGANIC BIPHASIC SYSTEM [J].

NAKANISHI, K ;

KIMURA, Y ;

MATSUNO, R .

BIO-TECHNOLOGY, 1986, 4 (05) :452-454

[9] CONTINUOUS SYNTHESIS OF N-(BENZYLOXYCARBONYL)-L-ASPARTYL-L-PHENYLALANINE METHYL-ESTER WITH IMMOBILIZED THERMOLYSIN IN AN ORGANIC-SOLVENT [J].

NAKANISHI, K ;

KAMIKUBO, T ;

MATSUNO, R .

BIO-TECHNOLOGY, 1985, 3 (05) :459-464

[10] A CHEMICAL MODIFICATION TO MAKE HORSERADISH-PEROXIDASE SOLUBLE AND ACTIVE IN BENZENE [J].

TAKAHASHI, K ;

NISHIMURA, H ;

YOSHIMOTO, T ;

SAITO, Y ;

INADA, Y .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1984, 121 (01) :261-265