LOCALIZATION OF THE ESTROGEN-BINDING SITE OF ALPHA-FETOPROTEIN IN THE CHIMERIC HUMAN RAT PROTEINS

被引：46

作者：

NISHI, S

MATSUE, H

YOSHIDA, H

YAMAOTO, R

SAKAI, M

机构：

[1] Department of Biochemistry, Hokkaido University, School of Medicine, Kita-ku, Sapporo 060

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 08期

关键词：

YEAST EXPRESSION SYSTEM; RECOMBINANT PROTEIN;

D O I：

10.1073/pnas.88.8.3102

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Rat alpha-fetoprotein (AFP) has been demonstrated to bind estrogen, whereas human AFP lacks the activity. We constructed four chimeric molecules from cDNAs encoding these AFPs with the use of two restriction sites common to them and expressed them as well as rat and human AFP cDNA in yeast. The recombinant molecules were purified, characterized, and found to have the predicted structures. Analyses of estrogen binding indicated that a rat AFP sequence composed of residues 423-506 that contains 31 rat-specific amino acids is essential for the activity.

引用

页码：3102 / 3105

页数：4

共 34 条

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