ADDITIVE ENHANCEMENT OF NEUTROPHIL COLLAGENASE ACTIVITY BY HOCL AND CATHEPSIN-G

被引：25

作者：

CHATHAM, WW ^{[1
]}

BLACKBURN, WD ^{[1
]}

HECK, LW ^{[1
]}

机构：

[1] DEPT VET AFFAIRS,BIRMINGHAM,AL

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 184卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)90626-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using preparations of latent collagenase derived from neutrophil specific granule extracts, the relative effects of cathepsin G and HOCl on activation of neutrophil collagenase were determined using a quantitative collagenase assay. Enhancement of collagenase activity by cathepsin G and physiologically relevant concentrations of HOCl were comparable, but HOCl mediated collagenase activation was reversible in the presence of HOCl scavenger. Collagenase activity in preparations treated with cathepsin G and HOCl simultaneously or sequentially was significantly greater than activity in preparations treated with HOCl or cathepsin G alone. The results indicated additive, yet independant enhancing effects of HOCl and cathepsin G on activity of neutrophil collagenase. © 1992.

引用

页码：560 / 567

页数：8

共 14 条

[1] SUBCELLULAR-LOCALIZATION OF THE B-CYTOCHROME COMPONENT OF THE HUMAN NEUTROPHIL MICROBICIDAL OXIDASE - TRANSLOCATION DURING ACTIVATION [J].