MOLECULAR CHARACTERIZATION OF 2 TYPES OF 22 KILODALTON ALPHA-ZEIN GENES IN A GENE-CLUSTER IN MAIZE

被引：12

作者：

LIU, CN ^{[1
]}

RUBENSTEIN, I ^{[1
]}

机构：

[1] UNIV MINNESOTA, INST PLANT MOLEC GENET, ST PAUL, MN 55108 USA

来源：

MOLECULAR AND GENERAL GENETICS | 1992年 / 234卷 / 02期

关键词：

EARLY IN-FRAME STOP CODON; GENE CLUSTER; MAIZE; MULTIGENE FAMILY; ZEIN;

D O I：

10.1007/BF00283845

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Five genes of the alpha-zein subfamily four (SF4) are located in a 56 kb genomic region of the maize inbred line W22. Their nucleotide and deduced amino acid sequences have been determined. The sequences define two types of alpha-zein SF4 genes - type 1 (T1) and type 2 (T2). The single TI alpha-zein SF4 gene codes for an alpha-zein protein with a M(r) of about 22 000. This is the first alpha-zein SF4 gene sequenced that contains no early in-frame stop codons in its coding sequence. The four T2 alpha-zein SF4 genes in this cluster contain one or two early in-frame stop codons. In addition, our TI and T2 genes differ markedly in the base sequences of their distal 5' non-translated flanking regions. The nucleotide and the deduced amino acid sequences of these two types of alpha-zein SF4 genes are similar (> 90 %) to one another and to all known alpha-zein SF4 genes and cDNAs. Of the known W22 alpha-zein SF4 genes, only one in six does not contain an early in-frame stop codon. If the number of alpha-zein SF4 genes is 15-20, then we estimate that only about 4 of the W22 alpha-zein SF4 genes are without in-frame early stop codons.

引用

页码：244 / 253

页数：10

共 55 条

[1] NUCLEIC-ACID SEQUENCE AND CHROMOSOME ASSIGNMENT OF A WHEAT STORAGE PROTEIN GENE [J].

ANDERSON, OD ;

LITTS, JC ;

GAUTIER, MF ;

GREENE, FC .

NUCLEIC ACIDS RESEARCH, 1984, 12 (21) :8129-8144

[2] UAG READTHROUGH DURING TMV RNA TRANSLATION - ISOLATION AND SEQUENCE OF 2 TRANSFER RNASTYR WITH SUPPRESSOR ACTIVITY FROM TOBACCO PLANTS [J].

BEIER, H ;

BARCISZEWSKA, M ;

KRUPP, G ;

MITNACHT, R ;

GROSS, HJ .

EMBO JOURNAL, 1984, 3 (02) :351-356

[3] ISOLATION AND SEQUENCING OF A 28 KD GLUTELIN-2 GENE FROM MAIZE - COMMON ELEMENTS IN THE 5' FLANKING REGIONS AMONG ZEIN AND GLUTELIN GENES [J].

BORONAT, A ;

MARTINEZ, MC ;

REINA, M ;

PUIGDOMENECH, P ;

PALAU, J .

PLANT SCIENCE, 1986, 47 (02) :95-102

[4] PRIMARY STRUCTURE OF A B1 HORDEIN GENE FROM BARLEY [J].

BRANDT, A ;

MONTEMBAULT, A ;

CAMERONMILLS, V ;

RASMUSSEN, SK .

CARLSBERG RESEARCH COMMUNICATIONS, 1985, 50 (06) :333-345

[5] A FUNCTIONAL SPLICE SITE IN THE 5' UNTRANSLATED REGION OF A ZEIN GENE [J].

BROWN, JWS ;

FEIX, G .

NUCLEIC ACIDS RESEARCH, 1990, 18 (01) :111-117

[6]

BROWN JWS, 1986, EUR J CELL BIOL, V42, P161

[7] PURIFICATION AND TRANSLATION OF ZEIN MESSENGER-RNA FROM MAIZE ENDOSPERM PROTEIN BODIES [J].

BURR, B ;

BURR, FA ;

RUBENSTEIN, I ;

SIMON, MN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (02) :696-700

[8]

CAMERONMILLS V, 1991, J CELL BIOCH A S, V15, P90

[9] APOLIPOPROTEIN B-48 IS THE PRODUCT OF A MESSENGER-RNA WITH AN ORGAN-SPECIFIC IN-FRAME STOP CODON [J].

CHEN, SH ;

HABIB, G ;

YANG, CY ;

GU, ZW ;

LEE, BR ;

WENG, SA ;

SILBERMAN, SR ;

CAI, SJ ;

DESLYPERE, JP ;

ROSSENEU, M ;

GOTTO, AM ;

LI, WH ;

CHAN, L .

SCIENCE, 1987, 238 (4825) :363-366

[10] LOCALIZATION OF SEQUENCES IN WHEAT ENDOSPERM PROTEIN GENES WHICH CONFER TISSUE-SPECIFIC EXPRESSION IN TOBACCO [J].

COLOT, V ;

ROBERT, LS ;

KAVANAGH, TA ;

BEVAN, MW ;

THOMPSON, RD .

EMBO JOURNAL, 1987, 6 (12) :3559-3564

← 1 2 3 4 5 6 →