PHENYLALANINE HYDROXYLASE OF HUMAN LIVER - ASSAY AND SOME PROPERTIES

被引：61

作者：

KAUFMAN, S

机构：

[1] Laboratory of Neurochemistry, National Institute of Mental Health, Bethesda

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1969年 / 134卷 / 01期

关键词：

D O I：

10.1016/0003-9861(69)90273-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive, reliable assay for phenylalanine hydroxylase in human liver extracts is described. A few milligrams of tissue are sufficient for a quantitative determination of the enzyme activity in normal liver. With this procedure, the apparent Km values for phenylalanine and for 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine, a cofactor analogue, have been determined for the human liver enzyme. © 1969.

引用

页码：249 / &

共 13 条

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