ESCHERICHIA-COLI PHOSPHOENOLPYRUVATE DEPENDENT PHOSPHOTRANSFERASE SYSTEM - COPURIFICATION OF HPR AND ALPHA-1-6 GLUCAN

被引：38

作者：

DOOIJEWAARD, G ^{[1
]}

ROOSSIEN, FF ^{[1
]}

ROBILLARD, GT ^{[1
]}

机构：

[1] STATE UNIV GRONINGEN,DEPT PHYS CHEM,GRONINGEN,NETHERLANDS

来源：

BIOCHEMISTRY | 1979年 / 18卷 / 14期

关键词：

D O I：

10.1021/bi00581a013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid, high-yield procedure has been developed for the purification of HPr from the Escherichia coli phosphoenolpyruvate dependent phosphotransferase system. During this procedure, the protein copurifies with a 2500-dalton homopolysaccharide which we have identified as α 1-6 glucan. The results of steady-state kinetic measurements of the phosphotransferase activity demonstrate that the polysaccharide works as an activator of the phosphotransferase system probably at the level of the HPr:P-E1 complex or the P-HPr:E11 complex. © 1979, American Chemical Society. All rights reserved.

引用

页码：2990 / 2996

页数：7

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