DIFFERENT UTILIZATION OF DEOXYCYTIDINE AS DNA-THYMINE IN LYMPHOID-TISSUES OF THE MOUSE

Small pieces of tissues were cut and homogenized in an appropriate volume of 2 M NaCl. Pure, highly polymerized DNA was extracted after RNase and protease treatment from the homogenate of lymphoid tissues of mouse. The extracted DNA contained no detectable RNA, and the yields ranged from 30% to 70% of DNA in the starting material. Using pure DNA extracted by this method, the rate of [G-3H]deoxycytidine incorporation into cytosine and thymine of DNA in thymus was different from that in mesenteric lymph nodes in mouse after administration of [G-3H]deoxycytidine. Thus, the utilization of deoxycytidine as DNA-thymine was differed between the thymus and mesenteric lymph nodes in the mouse. © 1978, Japan Society for Cell Biology. All rights reserved.