STUDIES ON SARGASSUM .3. QUANTITATIVE ULTRASTRUCTURAL AND CORRELATED PHYSIOLOGICAL STUDY OF THE BLADE AND STIPE ORGANS OF SARGASSUM-FILIPENDULA

Cytological comparisons of homologous tissues in blades and stipes by stereological analysis have shown differences exist between blade and stipe organs in Sargassum. Based on measurements of total thylakoid and cristae membrane surface areas in these organs blades were found to contain 61% more thylakoid membrane surface and 65% more cristae membrane than stipes on a per unit volume basis. Assuming photosynthesis and respiration are directly related to the surface area of the internal membranes in the respective organelles it is possible to predict that blades will have a 61% greater photosynthetic and a 65% greater respiratory potential. Photosynthetic and respiratory rates for blades and stipes were determined manometrically and show a 62% greater photosynthetic and 59% greater respiratory rates for the blade tissues agreeing very well with predicted values. Present evidence indicates that photosynthetic and respiratory rate differences observed in the blades and stipes in Sargassum are the result of increased membrane surface areas in the larger cells of the tissues which make up the blade. The basic cell structure, i.e., the percent volume of cell cytoplasm occupied by each organelle, is similar in homologous tissues of both organs regardless of cell size. Therefore physiological differences between the two organs are primarily due to changes in cell size and not in basic cell construction. This provides an interesting mechanism for producing physiological differences without changing basic cell structure in the organs of this plant. © 1979 Springer-Verlag.