EFFECTS OF MEDIUM COMPOSITION ON MF-ALPHA-1 PROMOTER-DIRECTED SECRETION OF A SMALL PROTEASE INHIBITOR IN SACCHAROMYCES-CEREVISIAE BATCH FERMENTATION

被引：7

作者：

CHEN, YP

KIRK, N

PIPER, PW

机构：

[1] UNIV LONDON UNIV COLL,DEPT BIOCHEM & MOLEC BIOL,LONDON WC1E 6BT,ENGLAND

[2] UNIV LONDON UNIV COLL,DEPT BIOCHEM ENGN,LONDON WC1E 6BT,ENGLAND

来源：

BIOTECHNOLOGY LETTERS | 1993年 / 15卷 / 03期

关键词：

D O I：

10.1007/BF00128309

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Casaminoacid supplementation of yeast nitrogen base (YNB) medium is widely used in the culture of recombinant yeasts as it enables respiratory growth on YNB and selection for URA3- or TRP1-bearing plasmids. Despite these advantages, this supplementation to YNB medium resulted in marked decreases in cellular productivity for the secretion of a protease inhibitor product in batch fermentation.

引用

页码：223 / 228

页数：6

共 19 条

[1] EXPRESSION, PURIFICATION AND CHARACTERIZATION OF RECOMBINANT HUMAN INSULIN-LIKE GROWTH FACTOR-I IN YEAST [J].