MOLECULAR-CLONING AND SEQUENCING OF THE GENE FOR A THERMOSTABLE N-CARBAMYL-L-AMINO ACID AMIDOHYDROLASE FROM BACILLUS-STEAROTHERMOPHILUS STRAIN-NS1122A

被引：14

作者：

MUKOHARA, Y

ISHIKAWA, T

WATABE, K

NAKAMURA, H

机构：

[1] Okawara Research Center, Nippon Soda Co., Ltd., 345 Takada, Odawara

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1993年 / 57卷 / 11期

关键词：

D O I：

10.1271/bbb.57.1935

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene for N-carbamyl-L-amino acid amidohydrolase was cloned from Bacillus stearothermophilus strain NS1122A into E. coli. This gene started with a TTG triplet and was predicted to encode a peptide of 409 amino acids, with a calculated molecular weight of 44,248. The deduced amino acid sequence shared moderate homology with that of the corresponding enzyme of Pseudomonas sp. strain NS671.

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页码：1935 / 1937

页数：3

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